Isolation and Antimicrobial Drug Susceptibility Profiling of Salmonella isolates from Raw Milk

Y. M. JALBANI, A. H. SHAH, G. S. BARHAM, N. A. KOREJO, Q. KALWAR, S. G. TUNIO

Abstract


The present study was conducted to determine the prevalence of Salmonella spp. in milk. The samples were collected from two intermediaries viz. farms (n=50) and retail shops (n=50) and antimicrobial susceptibility of the isolates were performed. The overall prevalence of Salmonella isolates at dairy farm was recorded as 28% amongst which 50% were S. typhi. Further, results revealed that eight dairy farms (80%) were positive, while two farms (20%) were detected as negative for Salmonella. The maximum prevalence was noted in Farm 7 (100%) whereas; milk samples from Farm 2 and Farm 10 were free from Salmonella contamination .None of the dairy shops found free of Salmonella carriage. The overall prevalence of salmonella was recorded as 44%. Among the salmonella isolates the prevalence of S.typhi was 54%. The maximum prevalence (60%) of Salmonella spp. in milk was noted in samples from five shops i.e. Shop 2, 4, 5, 9 and 10. More so, S. typhi was detected in 70% of milk samples from all seven shops except Shop 3, 6 and 9 which showed maximum carriage as 100%. Whereas, samples from three shops viz. Shop 1, 2 and 7 were free of S. typhi contamination. The organisms were found highly resistant to Neomycin and Kanamycin (84.21%) followed by Oxytetracycline (78.95%), Tetracycline (73.68%), Gentamycin (68.42%), Cefixime (52.63%), Cefoxitin (47.37%), Ciprofloxacin (36.84%) and Cefipime (32.11%). The overall MDR profile showed 68.4% S. typhi isolates were resistance to three and/or above antibiotics. A total of 26.31%, 21.04%, 15.78 and 5.26%, S.typhi showed resistance against three, four, five and six antimicrobial agents, respectively.


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