Spectrophotometric and Fluorometric Studies of cobalt(II), zinc(II), nickel(II), iron(II) and copper(II) of Pyruvic acid 4-phenyl-3-thiosemicarbazone

F. M. KHOKHAR, T. M. JAHANGIR, M. Y. KHUHAWAR, M. I. KHASKHELI, L. A. K. KHOKHAR

Abstract


The reagent pyruvic acid 4-phenyl-3-thiosemicarbazone (PAPTSC) was prepared by simple condensation reaction between pyruvic acid and 4-phenyl-3-thiosemicarbazide and characterized by Fourier transform-infra red (FT-IR), Ultraviolet-visible (Uv-vis)spectroscopy, mass spectrometry and thermal analysis. The reactions of the reagent towards cobalt(II),zinc(II), nickel(II), iron(II)and copper(II) were examined spectrophotometrically and spectrofluorometrically. Experimental conditions were optimized in terms of reaction time, solution stability, pH and concentration of reagent per determination for maximum absorbance. Factors affecting fluorescence intensities were also optimized. Linear calibration range obeying Beer’s law was within 5-25μg/mL with molar absorptivity 0.119-0.171×104L.mol-1.cm-1 and fluorescence intensities of emission were within 666-724 nm with excitation within 332-361 nm respectively. The highly stable complexes once formed did not show any change in absorbance up to 24 hours. The method was used for the determination of copper from different copper wires and iron and zinc from pharmaceutical preparations.


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