High Performance Liquid Chromatographic Method for Simultaneous Determination of Alprazolam with Antihistamines in Bulk Drug, Pharmaceutical Formulation and Human Serum

S. AKRAM, S. N. ALI, A. QAYOOM, S. IQBAL, N. NAZ, I. MEMON

Abstract


A high performance liquid chromatographic method with UV detection for simultaneous quantification of alprazolam with antihistamine was developed and validated following the ICH guidelines. Separation was achieved on Shimadzu Shim-pack CLC-ODS (M) 25 M column employing mobile phase 80:20methanol-water with pH 3.5 at 230 nm adjusting flow rate 1.0 mL min-1. The linear concentration ranges were 0.2-12.5 μg mL-1 for alprazolam and 0.4-25 μg mL-1 for antihistamines with correlation coefficient greater than 0.998 and detection limits 2.85, 6.92, 10.56, 4.9 ng mL-1 for promethazine, levocetirizine, alprazolam, and loratadine respectively. Validation showed RSD < 2% and 98.05-101.89% recovery. Proposed method was successfully applied for quantification of studied drugs in pharmaceutical formulations without excipients interference and also in human serum.

Full Text:

PDF

Refbacks

  • There are currently no refbacks.


Copyright (c) 2017 Sindh University Research Journal - SURJ (Science Series)

 Copyright © University of Sindh, Jamshoro. 2017 All Rights Reserved.
Printing and Publication by: Sindh University Press.