LIQUID CHROMATOGRAPHIC DETERMINATION OF DOPAMINE, METHYLDOPA, L-DOPA AND TYROSINE IN PHARMACEUTICAL PREPARATIONS USING 4- DIMETHYLAMINEBENZALALDEHYDE AS A DERIVATIZING REAGENT

A. A. MAJIDANO, N. A. SODHO, G. N. RAJPER, M. ALAMGIR, M. Y. KHUHAWAR

Abstract


Analytical procedure has been developed for the determination of Dopamine (DA) methyl dopa (MD) L-dopa (LD) and Tyrosine (TY) by High Performance Liquid Chromatography using 4- Dimethylaminebenzalaldehyde as pre-column derivatizing reagent, The separation was achieved from Phenomenex C – 18, 5 μm (150×4.6mm id) column when eluted isocritically with methanol-acetonitrile-water (58;3;39v/v/v with a flow rate of 1ml/min. The detection by UV was at 280 nm. The linear calibration curves were obtained within the range of 2-10 μg/ml with limit of detection within 0.065-0.098, 0.062-0.075 and 0.081 μg/ml for each of the compound. The derivatization , separation and quantitation was repeatable with relative standard deviations (RSDs) within2.3-3.8%(n=5).The method was applied for the determination of dopamine methyldopa, L-dopa and tyrosine from pharmaceutical preparations with RSDs within2.4-4.1% (n=3) and the results agreed with labeled values. The pharmaceutical preparations were also analyzed by standard addition and recovery of analytes was calculated within 95-98% with RSD 4.1%. The pharmaceutical additives did not affect the determination.

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