MIRNAS AS POTENTIAL TUMOR MARKERS FOR EARLY DIAGNOSIS OF BREAST CANCER

F. NADEEM, Q. JAMAL, M. HANIF

Abstract


In females, the cancer of the Breast is the most oftenly diagnosed cancer. At present, miRNA emerges as potential tumor marker in many cancers including breast cancer (BC). These miRNAs are actually a specific group of short sequenced RNA which are non-coding. The present study was conducted to identify the level of expression of miR-497 in patients with breast cancer along with it’s correlation with clinicopathologic features. A sample of 50 diagnosed cases of BC and 30 controls were selected. Blood samples were collected by venepuncture. Plasma was used for extraction of total RNA and cDNA was synthesized by using oligonucleotide primers for miRNA 497. Expression status of miR-497 was detected by RT-PCR. The results revealed that level of expression of miR-497 down regulated among 39 cases and 06 controls and had significant correlation with higher differentiation grade, metastasis in lymph node and late clinical stage (p= 0.001). Taken together, miR-497 might be used as possible marker of tumor for diagnosis as well as early detection of BC.

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