SCREENING OF LIPASE PRODUCING FUNGI AND CATALYTIC ACTIVITY FROM MOLASSES CULTURE MEDIUM

S. H. NAQVI, M.Y. KHAN, M. RAFIQ, M. U. DAHOT

Abstract


In present study, some fungal species Aspergillus fumigatus, Aspergillus niger, and Mucor geophillus grown on agro-industrial by product 2.5% and 5% molasses contained  mineral  medium  as a carbon  source  to  enhance  economic,  cheapest attractiveness in  submerged  culture  condition  of  commercially important enzyme lipase. These three species of fungi were screened on the basis of production of greater yield of lipase. It was evaluated maximum production of lipase 44.56 Units/ml by Mucor geophillus, when it was grown on 5% molasses mineral medium at 72 hours, which was found dominated as compared to other used species of fungi. Final pH, reducing sugar and total protein correlation with the yield of lipase were also checked from the fermented medium.


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