DETERMINATION OF MOLECULAR WEIGHT OF KERATIN IN HUMAN EPIDERMIS OF NORMAL AND PSORIATIC PATIENTS BY GEL FILTRATION

S. M. MAHESAR, M. U. DAHOT, M. Y. KHUHAWAR

Abstract


The molecular weights of protein polypeptides obtained from normal and psoriatic skin samples after purification and isolation of their fractions were determined. The polypeptide chains compositions in human epidermis have shown that characteristic modifications of fibrous proteins occur during normal epidermal keratinisation. The polypeptide chains compositions in psoriatic scales differ from that of normal stratum corneum. The difference in between normal and psoriasis involved skin is probably due to the defect in synthesis of alpha- chain. The epithelial protein fractions with low molecular weight keratins in epidermis was determined by Gel filtraration methods. Chromatography of these fractions to ascertain the accuracy and quantification of such proteins revealed the severity of psoriasis as inflammatory diseases. The protein markers of various molecular weights were used as standards. The keratin pattern of a given epithelial tissue may be modulated to some extent in the course of reactive changes, frequently resulting in higher complexity of keratin composition. In normal and psoriatic male and female subjects the molecular weight of isolated protein fractions ranged as 66 KD, 73KD, 68 KD and 69 KD respectively. Average molecular weight of purified protein fractions in normal and psoriatic male and female subjects was 60 ± 4, 44 ± 6, 52 ± 4 and 46±33 respectively. It has been noted that the a variations occurred in both of the genders of normal and psoriatic patients.


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